![Figure 3. Effect of scFv on sickle RBC adhesion to immobilized TSP. Parallel plate flow chambers were coated with purified TSP (2 μg/cm2, blocked with 2% BSA, and incubated with bacterial supernatants containing scFv. Control scFv (Ctrl scFv [a-hTG], n = 12), TSP-A10 (A10, n = 6), TSP-C5 (C5, n = 6), TSP-C6 (C6, n = 6), TSP-D2 (D2, n = 7), TSP-E7 (E7, n = 6), TSP-E9 (E9, n = 7), or control buffer (buffer, n = 19). Washed sickle RBCs were perfused through flow chambers at a wall shear stress of 1 dyne/cm2 as described in “Materials and methods.” After rinsing, adherent RBCs per unit area were counted by direct microscopic visualization. The results are shown as the means ± SEs of sickle RBC adhesion, normalized to sickle RBC adhesion to buffer-treated TSP (900 ± 110 RBCs/mm2,n = 19). The * indicates a P value of .001 or less compared with control scFv.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/2/10.1182_blood-2002-11-3497/6/h81434646003.jpeg?Expires=1725053302&Signature=xBGWJ5QowDgG6IM2N2N7hphtb5NcmnlturgdTKwobZfroT63S-3rG1mbOVfLWHEekNubm-QMcbnYBwm5O9z8NRS93xqsZYFVcAy~eeGofOPvyNMd4pFOVTFA-7QNxa6w6pUIkhts6jwPY1t~O5x4uu3-MAq5uoY4jFxM3lcBLyf5CBYVEUiFtdtJEi50ZlnczLfseSoNgObTt7zMHArXB2-EqJMGs5qSFZpCJHWOSiGwy-rwmNunsu87Dv-2yC2vjDzrybGC1ZSp2GCilVvQ8cuEjGN3DafhoHV98B3I3Kj8lvVpsO~WZ5h2BpasphY9HLH4ye7ibnP1GQLb7lH2-w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of scFv on sickle RBC adhesion to immobilized TSP. Parallel plate flow chambers were coated with purified TSP (2 μg/cm2, blocked with 2% BSA, and incubated with bacterial supernatants containing scFv. Control scFv (Ctrl scFv [a-hTG], n = 12), TSP-A10 (A10, n = 6), TSP-C5 (C5, n = 6), TSP-C6 (C6, n = 6), TSP-D2 (D2, n = 7), TSP-E7 (E7, n = 6), TSP-E9 (E9, n = 7), or control buffer (buffer, n = 19). Washed sickle RBCs were perfused through flow chambers at a wall shear stress of 1 dyne/cm2 as described in “Materials and methods.” After rinsing, adherent RBCs per unit area were counted by direct microscopic visualization. The results are shown as the means ± SEs of sickle RBC adhesion, normalized to sickle RBC adhesion to buffer-treated TSP (900 ± 110 RBCs/mm2,n = 19). The * indicates a P value of .001 or less compared with control scFv.
