scFvs specifically bind TSP. Bacterial supernatants containing expressed soluble scFv (TSP-A10, -C5, -C6, -D2, -E7, -E9, or anti–human thyroglobulin (a-hTG) control scFv that binds hTG were incubated in microtiter wells coated with 0.5 μg/well of purified human TSP-1 (TSP, ▦) or control protein hTG (□). Bound scFvs were detected by ELISA using anti–c-myc mAb 9E10 as described in “Materials and methods.” The graph depicts the means ± SEs of 4 experiments.