Type I/II IFN production by early life DCs in response to different stimuli. (A) IFN-γ production by 8 × 10⁴ purified splenic CD11c⁺ DCs in HL-1 medium containing GM-CSF and IL-4, in response to 5 ng/mL IL-12. Purity of cells was 96% for day-7 DCs and 97% for adult DCs. (B) IFN-α production by C57BL/6 DCs stimulated with CpG ODN (10 μg/mL) alone or in combination with poly I:C (100 μg/mL) for 24 hours. Supernatants were harvested and analyzed by ELISA. One of 3 similar experiments is shown.