IL-12p70 production by neonatal CD8α^- and CD8α⁺ DC subsets. Magnetically purified CD11c^high DCs from adult or 7-day-old BALB/c mice were sorted as CD8α^- and CD8α⁺ DC subsets by flow cytometry (purity was > 99% for all DC subsets). The indicated cell number was stimulated with 10 μg/mL CpG-ODN or left untreated, and 24-hour supernatants were tested for IL-12p70 content by ELISA. Data are representative of 2 experiments.