Early-life DCs display a distinct pattern of cell surface markers compared with adult DCs. (A) CD11c^high total cells (left) and CD11c^highCD4^-CD8^- double-negative cells (DN DC, right) as defined in Figure 3, were FACS analyzed for the expression of CD11b, 33D1, CD205, and CD1d. DCs from 7-day-old mice (gray histogram) and adult mice (bold line) were compared. M1 gate for each marker delineate positive cells in reference to control antibody (dotted line) and the percentage of positive 7-day-old DCs of the right column are shown. (B) At various ages, CD11c^high total cells are analyzed by FACS for CD11b, 33D1, CD205, and CD1d as in the left column in panel A.