Figure 5.
Analysis ofBandeiraea simplicifolia IB4 (BS lectin) binding to α-gal epitopes on WT lymphocytes as measured by flow cytometry. BS lectin (10 μg/mL) binding to spleen lymphocytes from (A) KO mouse, (B) WT mouse, (C-D) 2 irradiated KO mice receiving 20 × 106 KO lymphocytes from PKM-immunized mice, 20 × 106 KO bone marrow cells, and 20 × 106 syngeneic WT lymphocytes; these 2 mice were immunized with PKM on days 14 and 21 and flow cytometry analysis was performed on day 28. Panels A and B show data from 1 representative mouse in each group of 5 mice; panels C and D show data from 2 representative mice in a group of 5.
