Induction of tolerance on memory anti-Gal B cells by WT lymphocytes. (A) Anti-Gal response in KO recipients of 20 × 106 lymphocytes that include memory anti-Gal B cells from PKM-immunized mice and 20 × 106 syngeneic WT lymphocytes (▵); 2 × 106 syngeneic WT lymphocytes (○); or no WT lymphocytes (•). The mice were immunized with PKM on days 14 and 21 after adoptive transfer. Each data point shows the mean ± SE (n = 10). Statistical analysis by Student t test indicated significant differences (P < .0001) between the • group and the other 2 groups at all serum dilutions. (B) Repetition of the tolerance induction study of panel A with tolerizing WT lymphocytes depleted of stroma cells and macrophages (ie, adherent cells). Data are from 4 mice in each group receiving 20 × 106 lymphocytes from PKM-immunized KO mice and 20 × 106 syngeneic WT lymphocytes depleted of adherent cells (○) or no WT lymphocytes (•). (C) Repetition of the tolerance induction study of panel A in KO recipients with intact immune systems. Data are from 4 nonirradiated KO recipients in each group receiving 20 × 106 lymphocytes from PKM-immunized KO mice and 20 × 106 syngeneic WT lymphocytes (○) or no WT lymphocytes (•).
