HA-3 phenotyping and genotyping of family Ho. All members except Ho32 and Ho85 (hatched symbols) are HLA-A1 positive. • (females) or ▪ (males) indicate strong lysis by HA-3–specific CTLs (phenotype positive); open symbols indicate no lysis (phenotype negative). Hatched symbols indicate HLA-A*0101^- individuals, which were not typed phenotypically. Material from Ho01 was not available. Genotyping was determined by PCR on genomic DNA as described in “Patient, materials, and methods.” A band in the left lane indicates the presence of the HA-3^T allele, while the HA-3^M allele is represented by a band in the right lane. The results of the genotyping are show as T/T (HA-3^T homozygous), T/M (HA-3^T/M heterozygous), or M/M (HA-3^M homozygous).