Figure 4.
Figure 4. (A) Effect of 17-AAG on the levels of HSP-70, Akt, c-Src, c-Raf-1, and S6K1. HL-60/Neo, HL-60/Bcl-2, and HL-60/Bcl-xL were treated with 2.0 μM 17-AAG for 48 hours. Cells were harvested, and HSP-70, Akt, c-Src, c-Raf-1, and S6K1 levels were determined in the cell lysates by immunoblot analysis with the use of specific antibodies. As the loading control, β-actin was used.

(A) Effect of 17-AAG on the levels of HSP-70, Akt, c-Src, c-Raf-1, and S6K1. HL-60/Neo, HL-60/Bcl-2, and HL-60/Bcl-xL were treated with 2.0 μM 17-AAG for 48 hours. Cells were harvested, and HSP-70, Akt, c-Src, c-Raf-1, and S6K1 levels were determined in the cell lysates by immunoblot analysis with the use of specific antibodies. As the loading control, β-actin was used.

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