Immunoselected LV-transduced resting T cells are mainly in G₁ phase. Resting T lymphocytes were cultured with 5 ng/mL IL-7 or 10 ng/mL IL-15 and transduced 96 hours after stimulation. After an additional 96 hours, cells were analyzed for ΔLNGFr expression and immunoselected, as described in “Materials and methods.” Expression of ΔLNGFr after transduction (A) and after immunoselection (B) is shown. The percentage of ΔLNGFr-positive cells is indicated. Only viable cells, as assessed by PI exclusion, were analyzed. (C) DNA and RNA contents of immunoselected cells were measured by 7AAD and PY staining, respectively. The percentage of cells in S/G₂/M phases is indicated. The R2 gate, encompassing the G₀/G₁ population, was used for further analysis. (D) Histogram of RNA staining of R2-gated cells plotted for PY staining to distinguish G₀ from G₁ cells. The percentage of cells in G₁ is indicated. One of 2 similar experiments is shown.