Analysis of mRNA expression for P2XR and P2YR subtypes in different human DC populations. Profiles of P2R expression for MoDCs, CD1a⁺ dermal DCs, CD1c⁺ PBDCs, and IL-3R⁺ PDCs were generated by extracting mRNA from highly purified DC populations (purity, 95%-99%). Conventional RT-PCR was performed as described in “Materials and methods.” A representative experiment of at least 3 different donors is shown (for CD1a⁺ dermal DCs, n = 2).