Migration of MoDCs, CD1a⁺ dermal DCs, CD1c⁺ PBDCs, and IL-3R⁺ PDCs to the chemokines CXCL12 and CCL21. Day 7 MoDCs were cultured for 24 hours in the absence or presence of 250 μM ATP or a combination of TNF-α, IFN-α, and PGE₂. CD1c⁺ PBDCs or IL-3R⁺ PDCs were cultured overnight in the presence of GM-CSF and IL-4 or IL-3, respectively. Emigrant CD1a⁺ dermal DCs were harvested from cultured dermal sheets. Migration of DCs (10⁴ DCs/well) toward chemokines was assessed in a transwell chemotaxis assay. Representative experiments of at least 4 different donors are shown (mean ± SEM of triplicates). □ indicates medium; ▪, CXCL12; and ▧, CCL21.