Survivin expression in dendritic cells. (A) Survivin expression in human monocyte-derived dendritic cells was analyzed by Western blotting. (B) Standard ⁵¹Cr-release assay was performed using autologous immature DCs and autologous DCs activated with TNF-α from a healthy HLA-A2–positive as well as the HLA-A2– and survivin–positive colon carcinoma cell line HCT 116 as targets for survivin-specific CTLs.