Survivin-specific CTLs recognize autologous dendritic cells transfected with RNA isolated from A 498 tumor cells. Autologous DCs from a healthy HLA-A2⁺ donor generated from peripheral blood monocytes were pulsed with the cognate survivin peptide (Sv) or electroporated with tumor RNA isolated from the survivin-expressing A 498 tumor cell line and were used as target cells in a standard ⁵¹Cr-release assay (▪, •). DCs pulsed with an irrelevant HIV peptide (HIV) or electroporated with EGFP in vitro transcript (IVT) were used as controls in the assay (⋄, ▵).