Induction of survivin-specific CTL responses in vitro using peptide-pulsed mature DCs as antigen-presenting cells. DCs generated from adherent PBMNCs in the presence of GM-CSF, IL-4, and TNF-α were pulsed with the synthetic peptide derived from the survivin protein and used to induce a CTL response in vitro. Cytotoxic activity of induced CTLs was analyzed in a standard ⁵¹Cr-release assay using T2 cells pulsed with the cognate survivin peptide (T2 + Sv, ⋄) or an irrelevant (adipophilin) peptide (T2 + Ad, □) as targets. E:T indicates effector-to-target ratio.