Survivin expression in human tumor cell lines. (A) RT-PCR using survivin-specific primers was performed to analyze the RNA expression of survivin in human tumor cell lines. β2-Microglobulin–specific primers were used as controls. (B) Western blot analysis using a monoclonal antibody against survivin protein. Peripheral blood mononuclear cells (PBMNCs) and purified B and T lymphocytes were included in the analysis of protein expression as controls (C).