Sensitivity of alloactivated T cells and BW5147 cells to NO. (A) Nitrite accumulation in the MLR culture supernatant was measured on day 3 (not shown) and day 5 in duplicate wells (results represent the mean values of pooled wells). (B) Standard allogeneic MLR assays were performed in the presence of varying concentrations of GSNO. Results shown represent means ± SEs of quadruple wells. (C) BW5147 cells were cultured for 2 days in the presence of varying concentrations of GSNO. Results shown represent means ± SEs of quadruple wells. Results are from 1 of 2 independent identically designed experiments.