Flow cytometry analysis for EGFP expression in transduced cell lines. With the vectors generated, 10 different cell lines were transduced. EGFP expression was determined by flow cytometry. Gating was set using untransduced control cells (A-C). The CMV promoter led to high-level expression in all cell lines tested (D-F), but expression from the targeted vector was restricted to myeloma cells (G versus H and I).