Figure 8.
Figure 8. Id+ B-lymphoma cells become annexin V+ when exposed to cloned Id-specific Th1 cells. Id+ F9 and Id- F55 B-lymphoma cells were incubated for 4 hours together with either an Id-specific Th1 cell clone (7A10B2), or magnetic bead–purified naive CD4+ T cells from a TCR-transgenic mouse (effector-target ratio [E/T] 2:1), followed by staining. Annexin-V staining of gated B220+ propidium iodide–negative lymphoma cells is shown as shaded histograms, open histograms represent annexin-V staining of lymphoma cells in the absence of T cells.

Id+ B-lymphoma cells become annexin V+ when exposed to cloned Id-specific Th1 cells. Id+ F9 and Id- F55 B-lymphoma cells were incubated for 4 hours together with either an Id-specific Th1 cell clone (7A10B2), or magnetic bead–purified naive CD4+ T cells from a TCR-transgenic mouse (effector-target ratio [E/T] 2:1), followed by staining. Annexin-V staining of gated B220+ propidium iodide–negative lymphoma cells is shown as shaded histograms, open histograms represent annexin-V staining of lymphoma cells in the absence of T cells.

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