Kinetic analysis of secondary changes in IL-4, IFN-γ, and GATA-3 expression in CD3⁺ T cells upon G-CSF treatment in vitro. Fold change in gene expression levels of IL-4, IFN-γ, and GATA-3 in CD3⁺ T cells was determined by quantitative real-time RT-PCR upon G-CSF treatment in vitro at the indicated time points. The mRNA expression was quantified by applying Taqman technology. Data are expressed as mean of 2 independent experiments from 2 healthy donors (n = 2).