Characterization of donor-derived DCs after transfer of purified CD8α^- DCs. Splenic CD8α^-CD11c^hiMHC 2^hi DCs were isolated and sorted from Ly5.2 mice and transferred intravenously into nonirradiated Ly5.1 recipients (5 × 10⁶ per mouse). After 3.5 days, DCs were isolated from the pooled recipient spleens, and Ly5.2⁺CD11c⁺ DCs were analyzed for CD8α expression. Histograms indicate Ly5.2 gated cells either unstained (gray line) or stained (black line) for CD8α, with the vertical line indicating the cutoff for levels of CD8α on CD8α^hi DCs. Of 7 transfer experiments, 4 gave no detectable donor-derived DCs, whereas 3 gave donor-derived DCs containing 5% to 33% CD8α⁺ DCs. The experiment presented is the one showing the highest donor cell recoveries and the highest proportion of Ly5.2⁺CD8α⁺ DCs.