Figure 2.
Figure 2. Intranodal vaccination with KLH-pulsed semimature DCs induces strong DTH reactivity to KLH. (A) Quantitation of the KLH-specific immune response by assessment of DTH reactivity to KLH in vivo. DTH reactivity to KLH was tested at week 0, 4, and 10 by intradermal injection of 5 μg KLH in 200 μL sterile PBS into the forearm. 48 hours after the challenge maximal diameter of the elicited indurated erythema was assessed. ▪ indicates patient 1; •, patient 2; ▴, patient 3; □, patient 4; and ○, patient 5. (B) Immunhistochemical characterization of the lymphocytic infiltrate at the DTH site. Alkaline-phosphatase-antialkaline phosphatase (APAAP) staining with a CD45RO mAb was performed, demonstrating a strong perivascular and epidermal infiltration of CD45RO+ memory T cells. Original magnification, × 100.

Intranodal vaccination with KLH-pulsed semimature DCs induces strong DTH reactivity to KLH. (A) Quantitation of the KLH-specific immune response by assessment of DTH reactivity to KLH in vivo. DTH reactivity to KLH was tested at week 0, 4, and 10 by intradermal injection of 5 μg KLH in 200 μL sterile PBS into the forearm. 48 hours after the challenge maximal diameter of the elicited indurated erythema was assessed. ▪ indicates patient 1; •, patient 2; ▴, patient 3; □, patient 4; and ○, patient 5. (B) Immunhistochemical characterization of the lymphocytic infiltrate at the DTH site. Alkaline-phosphatase-antialkaline phosphatase (APAAP) staining with a CD45RO mAb was performed, demonstrating a strong perivascular and epidermal infiltration of CD45RO+ memory T cells. Original magnification, × 100.

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