Mediation of BD-FANCF interactions by conditionally expressed HA-FANCG in the 3-hybrid system. The BrFG construct was paired with constructs expressing the indicated AD-fusion proteins by mating haploid yeast strains. HA-FANCG is expressed from the BrFG construct in the absence of methionine (□) but repressed by the presence of 4 mM methionine in the media (▦). The BD-FANCF fusion protein is constitutively expressed, as are all AD fusions. Activation of the LacZ reporter gene was measured in AH109/Y187 diploids by liquid β-galactosidase assay. β-Galactosidase activity units were calculated as in Figure 1. The results shown indicate the mean and SE for 3 (+Met) or 4 (-Met) assays, each done in triplicate.