Mapping binding sites for the interactions of FANCG with FANCA and FANCF. (A) Mapping the FANCG binding region on FANCA. (B) Mapping the FANCA binding region on FANCG. (C) Mapping the FANCG binding region on FANCF. (D) Mapping the FANCF binding region on FANCG. Yeast strain PJ69-4A was cotransformed with the expression constructs indicated. Constructs activating the reporter genes above background levels are shaded. The dotted lines represent internal deletions. (++), (+), and (-) indicate good, weak, and absent growth, respectively. β-Galactosidase activity units in the liquid ONPG assay were calculated as 1000 × OD₄₂₀ divided by the product of the volume (milliliter) × time (minutes) × OD₆₀₀. The values shown indicate the mean and SE for 4 assays, each done in triplicate. Nd indicates that no β-galactosidase assay was performed because of nonactivation of the ADE2 and HIS3 reporters. NLS indicates nuclear localization signal; LZ, leucine zipper.