HOX11 mutants are expressed in nuclei of transfected cells. Transiently transfected HEK 293T cells were cultured on fibronectin-coated coverslips and fixed in 3.7% formaldehyde. Immunohistochemical analysis was performed using 10 μg/mL anti-HOX11 polyclonal antibody and 5 μg/mL fluorescein-linked goat–anti-rabbit secondary antibody and visualized using a fluorescence microscope. Original magnification, × 100. (A) pcDNA3 vector, (B) HOX11 WT, (C) HOX11 H3d, (D) HOX11 M5, (E) HOX11 Thr47Ile, (F) HOX11 Lys55Gln.