Figure 1.
Figure 1. HOX11 represses basal transcription of TATA-containing and TATA-less promoters in HeLa cells and C2C12 cells. (A) Transcriptional repression by HOX11 is concentration dependent. Transient transfection and CAT assays were performed in C2C12 cells and HeLa cells using pSV2CAT (2 μg) and indicated amounts of pcDNA3-HOX11. Relative CAT activity, expressed as percentage of control, is indicated. Data shown are representative of 3 independent experiments. (B) HOX11 represses transcription from different promoters. pcDNA3-HOX11 (8 μg) and reporter plasmids (2 μg) containing HSV tk (pBLCAT2), CMV (pCMVCAT), SV40 early, and rat neu (pneuCAT) promoters were cotransfected into C2C12 cells. CAT activity expressed as percentage of control. (C) HOX11 represses SV40 promoter activity in murine cells. pcDNA3-HOX11 (8 μg) and pSV2CAT (2 μg) were cotransfected into NIH3T3 cells and CAT activity measured.

HOX11 represses basal transcription of TATA-containing and TATA-less promoters in HeLa cells and C2C12 cells. (A) Transcriptional repression by HOX11 is concentration dependent. Transient transfection and CAT assays were performed in C2C12 cells and HeLa cells using pSV2CAT (2 μg) and indicated amounts of pcDNA3-HOX11. Relative CAT activity, expressed as percentage of control, is indicated. Data shown are representative of 3 independent experiments. (B) HOX11 represses transcription from different promoters. pcDNA3-HOX11 (8 μg) and reporter plasmids (2 μg) containing HSV tk (pBLCAT2), CMV (pCMVCAT), SV40 early, and rat neu (pneuCAT) promoters were cotransfected into C2C12 cells. CAT activity expressed as percentage of control. (C) HOX11 represses SV40 promoter activity in murine cells. pcDNA3-HOX11 (8 μg) and pSV2CAT (2 μg) were cotransfected into NIH3T3 cells and CAT activity measured.

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