Figure 3.
![Figure 3. Sphingolipids inhibit T-cell proliferation with different dose responses. T cells were either left intact (□, left column) or were stimulated with 1 μg/mL plated anti-CD3 plus 1 μg/mL anti-CD28 (▪), for 3 days in the presence or absence of different concentrations of S1P, DHS1P, ceramide (CER), or sphingosine (SPH), in media supplemented with 10% serum. The incorporation of [3H]thymidine was done as in Figure 2. P values comparing T-cell proliferation in the presence of sphingolipids to the proliferation in their absence are placed on top of each column. Data are representative of 3 experiments performed. Error bars indicate means ± SD; NS, not significant.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/12/10.1182_blood-2002-09-2962/6/h81234468003.jpeg?Expires=1726834709&Signature=m9zgYEhfXV-Nyc8HSkJj~TN80pX-SUC2BSiyzd805z4cVkQvQpEt0RptMucR5q9zHhu5lrgOWjxB~yHFf-zNp7k7cOpPEDjDrANbsn-5AvxYqkv4ar2tAxyNv-JEI~yxe0J8AqFGtIT-lcOmOPMlvNGWRJJq2S8~Nbth7QXqY-DxBG34Y-c3UqQdNYYsGmekbKLKwctBM6K0~7YMY5OqjRNrSajDzVYwEUkeXOv6KxY76aEGjnVC2Fv7db3YW9oNqqY602FwOs-bJi0LsAQ-wDKoIcO6EyRtP9kjdFacYaNW66eZMO6W3sVbGL~gG6wSof6HDXgiZz3XZMGh04fX~w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Sphingolipids inhibit T-cell proliferation with different dose responses. T cells were either left intact (□, left column) or were stimulated with 1 μg/mL plated anti-CD3 plus 1 μg/mL anti-CD28 (▪), for 3 days in the presence or absence of different concentrations of S1P, DHS1P, ceramide (CER), or sphingosine (SPH), in media supplemented with 10% serum. The incorporation of [3H]thymidine was done as in Figure 2. P values comparing T-cell proliferation in the presence of sphingolipids to the proliferation in their absence are placed on top of each column. Data are representative of 3 experiments performed. Error bars indicate means ± SD; NS, not significant.
