c-met and VE-cadherin are inversely regulated at the healing edge in wound assay. Confluent HUVEC monolayers were damaged by scratching a linear wound and incubated in regular medium. At the indicated times cultures were fixed and processed for double-staining immunofluorescence analysis of c-met and VE-cadherin expression at the border of the wounded area. Data are representative of 3 experiments. Images in far left column are brightfield images. For all images in Figure 2: original magnification, × 400.