Injection of αDEC-OVA activates Treg in vivo. (A) CD25⁺ T cells from reconstituted mice that were injected with αDEC-OVA or Gl117-OVA were isolated and mixed with B6-derived CD4⁺ T cells at different ratios. Cells were then stimulated with Balb/c-derived BMDCs, and T-cell proliferation was assayed by ³H-thymidine incorporation. Pure B6 T cells served as positive control. The asterisk indicates a significant difference (experiments were carried out 5 times; mean of triplicate wells; α < 0.05) from proliferation obtained with T cells only. (B) Tissue-culture supernatants from cocultures of T cells described in panel A (as indicated) were added to conventional MLR, and T-cell proliferation was assayed by ³H-thymidine incorporation (mean of triplicate wells ± SD is shown).