Induction of apoptosis by inhibition of MAPK and mTOR pathways. 32D cells were untreated (lane 1) or were treated with 10 μM UO126 (lane 2), 5 μM rapamycin (lane 3), or 5 μM rapamycin in combination with 10 μM UO126 (lane 4). ELISA was used to measure apoptosis after 24 hours of treatment. Data represent the mean ± SD of quadruplicate determinations. This experiment was repeated at least 3 times with slightly variable concentrations of inhibitors.