Effect of MPA treatment on Akt and JNK phosphorylation. 32D and FL5.12 cells were treated with 2 μM MPA alone or MPA plus 100 μM guanosine and were harvested at indicated time points. Western blot analysis for phospho-Akt and total Akt was performed on 32D cells (A), and for phospho-JNK and total JNK was performed on 32D (B) and FL5.12 cells (C). Total lysate (40 μg) was used for these blots.