Effect of MPA on 32D, FL5.12, and BaF3 cell growth and survival. Cells were treated with indicated doses of MPA for 24 hours. (A) 32D cell counts determined in quadruplicate. This is a representative experiment that has been repeated in 32D cells at least 3 times; the same effect was also seen in FL5.12 and BaF3 cells. (B) 32D, BaF3, and FL5.12 cells were treated with indicated doses of MPA for 24 hours, and apoptosis was measured by the presence of fragmented DNA using ELISA. Values are mean ± SD of 3 independent experiments for each cell line. (C) Apoptosis of 32D cells as measured by DNA ladder formation. 100-bp DNA markers (MWt) are included.