Expression of Sca-1 and c-kit on lineage-depleted mBM cells. Expression of these markers was used to form gates and sort Sca-1⁺c-kit⁺lin^— cells (upper-right quadrant) and Sca-1⁺c-kit^—lin^— cells (upper-left quadrant) for further expression and functional analysis of populations of cells. Sample dot plot shown is representative of data obtained from 6 independent mBM samples.