Figure 6.
![Figure 6. Pulse-chase analysis of normal and GPIIb mutants. Autoradiography of immunoprecipitated GPIIb pulse-labeled and chased with [35S]methionine and cold methionine, respectively. (A) Total GPIIb precipitated by SZ22 in the absence of CaCl2. (B) GPIIb complexed with GPIIIa precipitated in the presence of CaCl2 by 10E5 for WT and by AP3 for GPIIb-alt.25 and GPIIb-del.20. The relative intensity of labeled bands was determined by densitometry and expressed as percent of maximum incorporation. ▪ indicates WT; ▵, alt.25; and ○, del.20.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/12/10.1182_blood-2002-08-2452/6/h81234454006.jpeg?Expires=1724242422&Signature=umIEAxwRmfmk4byjzvS1XoE-lbCog0eweDpEaY~vOX7w7Bs4D66KY3onaecUWRvhsXUFCV80yHPGn077stJgXskHjbRL4HA~uUbgITWj2crXbej~iiZnaoQik1nBwCnCTUDeH4hQKVRRRKc55ZaZvoaFZfrIqAxfyHShmXJIUitqWsBKNF5mblSmZCwbiG8~uYNvKWbeL150Wrv5b1NgzjiVbNk46lM6j32I9oDN9G0OLRXbeoKUxcjMGBHaNXiPpdIDWAcrTeufFgb1HLxAdeuTQOq4-xzPkZUomI3K4bRhKz27lsgG-4efNi8e4AUbztFqshEHcYsSWlr0nSwbiw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse-chase analysis of normal and GPIIb mutants. Autoradiography of immunoprecipitated GPIIb pulse-labeled and chased with [35S]methionine and cold methionine, respectively. (A) Total GPIIb precipitated by SZ22 in the absence of CaCl2. (B) GPIIb complexed with GPIIIa precipitated in the presence of CaCl2 by 10E5 for WT and by AP3 for GPIIb-alt.25 and GPIIb-del.20. The relative intensity of labeled bands was determined by densitometry and expressed as percent of maximum incorporation. ▪ indicates WT; ▵, alt.25; and ○, del.20.
