Figure 1.
Figure 1. FACS analysis of transfected cells. Open histograms show the results from BHK cells expressing normal GPIIIa that were transfected with cDNA of normal GPIIb (WT) or mutated GPIIb (del.20 or alt.25). Monoclonal antibodies against GPIIb (SZ22), GPIIIa (AP3), and GPIIb/IIIa (10E5) were used. FITC-antimouse IgG plots are shown as black histograms. WT cells exhibited surface expression of GPIIb, GPIIIa, and GPIIb/IIIa, whereas cells containing GPIIb-del.20 or GPIIb-alt.25 displayed neither GPIIb nor GPIIb/IIIa but did display GPIIIa complexed with hamster αv.

FACS analysis of transfected cells. Open histograms show the results from BHK cells expressing normal GPIIIa that were transfected with cDNA of normal GPIIb (WT) or mutated GPIIb (del.20 or alt.25). Monoclonal antibodies against GPIIb (SZ22), GPIIIa (AP3), and GPIIb/IIIa (10E5) were used. FITC-antimouse IgG plots are shown as black histograms. WT cells exhibited surface expression of GPIIb, GPIIIa, and GPIIb/IIIa, whereas cells containing GPIIb-del.20 or GPIIb-alt.25 displayed neither GPIIb nor GPIIb/IIIa but did display GPIIIa complexed with hamster αv.

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