Detection of binding proteins to the TME by EMSA. (A) Sense sequences of the double-stranded oligonucleotides used in EMSA. TME indicates the wild-type TME sequence. Mutant oligonucleotides, Mut-1 to Mut-7, have various mutations in the TME and were used as the competitors. Bold underlined text indicates mutated sequences. (B) Wild-type TME EMSA performed with 6 μg nuclear extracts from 4 cell lines. (C-D) Competition experiments performed with nuclear extracts from HEL (C) and HepG2 (D) cells. (–) indicates no competitor. Arrows indicate the specific competed bands.