Mac-1 requirement in Ab-mediated antimelanoma immunity.

The effect of mAb TA99 and peg–G-CSF on melanoma growth was studied in WT and Mac-1^−/− mice. Upon tumor inoculation, mice were treated with saline or TA99 combined with or without peg–G-CSF. (A) Lungs were excised at day 21 to analyze surface metastases. (B) Numbers of metastases in saline-treated (■) or TA99-treated mice (▪) combined without (i) or with (ii) peg–G-CSF were quantified. Results represent means ± SEMs from 2 individual experiments (WT: n = 14; Mac-1^−/−: n = 12). P values of significant differences were determined using unpaired Welcht tests.