Figure 2.
Figure 2. SDS-PAGE and Western blotting of factor VIIIa cleavage reactions. Human factor VIIIa (250 nM) was incubated with APC derivatives (40 nM) on 100 μM PC/PS vesicles in 5 mM Ca2+ for 5 minutes at 37°C. Following electrophoresis, proteins were transferred to nitrocellulose membranes and Western blotted by either the 58.12 anti-A1 subunit monoclonal antibody (A) or by the R8B12 anti-A2 subunit monoclonal antibody (B).

SDS-PAGE and Western blotting of factor VIIIa cleavage reactions. Human factor VIIIa (250 nM) was incubated with APC derivatives (40 nM) on 100 μM PC/PS vesicles in 5 mM Ca2+ for 5 minutes at 37°C. Following electrophoresis, proteins were transferred to nitrocellulose membranes and Western blotted by either the 58.12 anti-A1 subunit monoclonal antibody (A) or by the R8B12 anti-A2 subunit monoclonal antibody (B).

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