Figure 3.
![Figure 3. The effect of low extracellular [Ca2+]i (50 nM) and thapsigargin on the APC-induced [Ca2+]i signal. (A) BECs in media containing 1.8 mM or 50 nM Ca2+ were subjected to APC (100 nM) perfusions. Traces represent [Ca2+]i of 12 to 15 single cells from a representative experiment. Horizontal line indicates the time of APC addition. (B) Thapsigargin (1 μM; TG) was introduced to BEC culture as indicated by the horizontal line, and APC (100 nM) was administered for 60 seconds at the time indicated by the arrow. Traces show [Ca2+]i in single cells from representative experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/12/10.1182_blood-2002-12-3680/6/h81234484003.jpeg?Expires=1724233472&Signature=WPJoWYANVmDVbhF8hTbEWmMJgfEVW4t1m9S6lvrNcLY-4BnRXNSFcG60LVpGqp-0b2vG8mMAXCIsF0rPvzoUeYCYlVEqYAZ3KqRU9ONcCvny6Ssi1s0DfptHf-WS6BWXwKr0EVBNEGFknbkznH9ZYHv1CVQH~m~43TVURom4CDJfQK68KO0b8n-dFYEy5t2S2Z7h4crCSmJpil5-cNUN~xr7~dR52Ovl7yIWa8Z9VDZVya72C69gxisiDr-QXzuYxeSh2N3VtT1~Amkc0klWsI2lr5HMqkVDgURp9tCQgWWLmv6uMnbn58dUyBkUBAsD3Rq56krwXHeV4TL--g1xKA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The effect of low extracellular [Ca2+]i (50 nM) and thapsigargin on the APC-induced [Ca2+]i signal. (A) BECs in media containing 1.8 mM or 50 nM Ca2+ were subjected to APC (100 nM) perfusions. Traces represent [Ca2+]i of 12 to 15 single cells from a representative experiment. Horizontal line indicates the time of APC addition. (B) Thapsigargin (1 μM; TG) was introduced to BEC culture as indicated by the horizontal line, and APC (100 nM) was administered for 60 seconds at the time indicated by the arrow. Traces show [Ca2+]i in single cells from representative experiments.
