Real time quantitative PCR (Q-PCR) analysis of mononuclear cells (MNCs) and CD34⁺cells from patients in CCR with imatinib mesylate treatment.BCR/ABL mRNA levels in MNCs and CD34⁺ cells were measured using a Q-PCR assay. Results were expressed as a ratio of BCR/ABL to the internal control gene GAPDH. Q-PCR results of BCR/ABL expression in CD34⁺ cells and MNCs for 8 patients are shown. Two patients did not have detectable BCR/ABL message in either total nucleated cells or CD34⁺ cells using this assay.