American Society of Hematology

Figure 5.

$Figure 5. ROS levels in RBCs. ROS levels of the various density fractions of RBCs from Prx II–/– (shaded histograms) and wild-type (open histograms) mice were determined by DCFH-DA staining followed by fluorescence-activated cell sorter (FACS) analysis. (A) Fluorescence intensity of total RBCs from Prx II–/– and wild-type mice. The panels B-G correspond to fractions H2 to H7 from the Prx II–/– mice and equivalent fractions W2 to W7 from the wild-type mice in Figure 4. The ROS levels in fractions H3 to H6 from the Prx II–/– mice were significantly increased compared with wild type. The x- and y-axes represent the number of cells and fluorescence intensity, respectively. Inset panels indicate negative controls of panels A-G, which DCFH-DA was not preincubated to each samples.$

ROS levels in RBCs. ROS levels of the various density fractions of RBCs from Prx II^–/– (shaded histograms) and wild-type (open histograms) mice were determined by DCFH-DA staining followed by fluorescence-activated cell sorter (FACS) analysis. (A) Fluorescence intensity of total RBCs from Prx II^–/– and wild-type mice. The panels B-G correspond to fractions H2 to H7 from the Prx II^–/– mice and equivalent fractions W2 to W7 from the wild-type mice in Figure 4. The ROS levels in fractions H3 to H6 from the Prx II^–/– mice were significantly increased compared with wild type. The x- and y-axes represent the number of cells and fluorescence intensity, respectively. Inset panels indicate negative controls of panels A-G, which DCFH-DA was not preincubated to each samples.

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