Morphologic phenotypes of Prx II^–/– mice. (A) Splenomegaly in Prx II^–/– mice and heterozygous mice at 8 weeks of age. (B-C) Prussian blue–stained sections of spleen from wild-type (B) and Prx II^–/– (C) mice. Red pulp was increased in Prx II^–/– mice. (D-F) Prussian blue–stained sections of liver from splenectomized wild-type mice (D), unsplenectomized Prx II^–/– mice (E), and splenectomized Prx II^–/– mice (F). Stained iron was markedly increased in the livers of splenectomized Prx II^–/– mice but scant in the livers of unsplectomized Prx II^–/– mice and absent from the livers of wild-type mice. Original magnifications: panels B-C, × 200; panels D-F, × 400.