Targeted disruption of the mouse Prx II gene. (A) The genomic structure of the wild-type Prx II gene (top) and the targeted locus (bottom) are shown. The recognition sites for BamHI (B), KpnI (K), EcoRI (E), and HindIII (H) restriction enzymes are indicated. Exons encoding Prx II are represented by black boxes. W1, W2, N1, and N2 indicate PCR primers. (B) PCR analysis of the genomic DNA isolated from wild-type (+/+), Prx II heterozygous (+/–), and Prx II homozygous (–/–) mice. The 700-bp and 250-bp arrows indicate the PCR products of Prx II^–/– and Prx II^+/+ mice, respectively. (C) Western blot analysis of Prx II expression in RBCs from wild-type, Prx II heterozygous, and Prx II^–/– mice.