Figure 3.
Figure 3. Arrest of flow platelets in whole blood on immobilized 4N1K and 4NGG peptides. Permanox Lab-Tek 1 chamber slides were coated with 50 or 100 μM 4N1K (a CD47 agonist from the CBD of TSP-1) or 4NGG (control) peptides and were placed in the flow chamber. Platelets were first labeled with calceine in PRP and then incubated or not incubated with 40 μg/mL anti-CD47 mAb B6H12 for 10 minutes at 37°C. Whole blood was reconstituted and perfused through the chamber at 37°C and at a shear rate of 100 seconds–1 for 3 minutes. Platelet adhesion, expressed as percentage of surface covered with platelets, is the average ± SEM of 10 random fields per coverslip. ***P < .001. This experiment is representative of 3 experiments performed using blood from different donors.

Arrest of flow platelets in whole blood on immobilized 4N1K and 4NGG peptides. Permanox Lab-Tek 1 chamber slides were coated with 50 or 100 μM 4N1K (a CD47 agonist from the CBD of TSP-1) or 4NGG (control) peptides and were placed in the flow chamber. Platelets were first labeled with calceine in PRP and then incubated or not incubated with 40 μg/mL anti-CD47 mAb B6H12 for 10 minutes at 37°C. Whole blood was reconstituted and perfused through the chamber at 37°C and at a shear rate of 100 seconds–1 for 3 minutes. Platelet adhesion, expressed as percentage of surface covered with platelets, is the average ± SEM of 10 random fields per coverslip. ***P < .001. This experiment is representative of 3 experiments performed using blood from different donors.

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