LF 15-0195 does not influence the postmitochondrial pathway to cell death.

(A-B) Cell-free extracts were obtained from Jurkat T cells exposed to either vehicle or LF 15-0195 for 3 days and treated for indicated times with a cytochrome c (5 μM)/dATP (1 mM) combination before studying caspase-3 cleavage by Western blot. The asterisk indicates the cleavage products (A) and caspase activities by using indicated peptide substrates (B). ■ indicates control cells; ▪, LF 15-0195–treated cells. Error bars indicate means ± SD. (C) Expression of cytochrome c, DIABLO/Smac, XIAP, mitochondrial Hsc70 (mt Hsc70), and Bcl-2 was studied by Western blot in the mitochondria and the S100 fractions of Jurkat T cells exposed for 3 days to either vehicle (−) or LF 15-0195 (+; 0.1 μM), then treated with CH11 Ab (25 ng/mL) for 80 minutes.