American Society of Hematology

Figure 5.

$Figure 5. Adhesion to immobilized PF4 decreases proliferation of cells giving rise to colony-forming units. Left pair of bars: CD34+ cells in suspension were incubated 16 hours either in the absence (▪) or presence (▦) of 10 μg/mL PF4 in solution (sPF4) and percent S-phase of total colony-forming cells (BFU-E, CFU-GM, and CFU-GEMM) analyzed by thymidine suicide analysis. Right 3 pairs of bars: CD34+ cells were cultured 16 hours in wells whose bottoms were coated with immobilized bovine serum albumin (BSA), poly-L-lysine (PLL), or platelet factor 4 (PF4). Adherent (A) and nonadherent (N) cells were then separated, and the fraction of total colony-forming cells in S-phase was quantified. Cells adherent to PF4 had significantly decreased proportion of cells in S-phase when compared with nonadherent cells (P < .005). Error bars represent SEMs.$

Adhesion to immobilized PF4 decreases proliferation of cells giving rise to colony-forming units. Left pair of bars: CD34⁺ cells in suspension were incubated 16 hours either in the absence (▪) or presence (▦) of 10 μg/mL PF4 in solution (sPF4) and percent S-phase of total colony-forming cells (BFU-E, CFU-GM, and CFU-GEMM) analyzed by thymidine suicide analysis. Right 3 pairs of bars: CD34⁺ cells were cultured 16 hours in wells whose bottoms were coated with immobilized bovine serum albumin (BSA), poly-L-lysine (PLL), or platelet factor 4 (PF4). Adherent (A) and nonadherent (N) cells were then separated, and the fraction of total colony-forming cells in S-phase was quantified. Cells adherent to PF4 had significantly decreased proportion of cells in S-phase when compared with nonadherent cells (P < .005). Error bars represent SEMs.

This Feature Is Available To Subscribers Only