Apoptosis induction of 12B1-D1 cells by Mit-C treatment.

(A) 12B1-D1 cells were treated with 100 μg/mL Mit-C for 1 hour, washed, and then cultured in complete media for an additional 6 hours. Induction of apoptosis was assessed by annexin V and PI staining. (B) DNA fragmentation analysis. DNA extracted from either 12B1-D1 cells (lane 1) or from 12B1-D1 cells that had been treated with Mit-C for 1 hour and recultured in complete media for additional 6 hours (lane 2) or 24 hours (lane 3).