Western blot analysis showing BRCA1 and BCR-ABL expression in human and murine clones expressing variable levels of BCR-ABL. (A) Levels of BRCA1 and BCR-ABL protein were analyzed by immunoblotting 50 μg whole-cell extracts of UT-7 cell clones (lane 1, UT-7/P; lane 2, UT-7/E8-1; lane 3, UT-7/E8-2; lane 4, UT-7/9). (B) Levels of Brca1 and BCR-ABL protein were analyzed by immunoblotting 50 μg whole-cell extracts of murine Ba/F3 clones (lane 1, Ba/F3; lane 2, Ba/F3 210). (C) Levels of Brca1 and BCR-ABL protein were analyzed by immunoblotting 50 μg whole-cell extracts of Tet^off conditional cell line (lane 1, cells exposed to doxycycline; lane 2, no exposure of cells to doxycycline).