Fig. 5.
Binding of α-spectrin tosphJ/sphJ and +/+ reticulocyte membranes.
Reticulocyte cultures from each were exposed sequentially for 15-minute intervals to [35S] methionine, unlabeled methionine, and [3H] leucine. During a 2-hour chase in unlabeled methionine and leucine, samples were removed, ghosts made, and the [3H]/[35S] ratios determined from gel-purified α-spectrin. Ratios presented are the averages of 3 experiments (P < .01).