Downstream effects of mutations.

(A) Northern blots of total RNA from spleen (lanes 1-3) and reticulocytes (lanes 4-6) of +/+ (lanes 1,4),sph^J/sph^J (lanes 2,5), andsph^2BC/sph^2BC (lanes 3,6) mice. UV shadowing was used to check RNA loading. (B) Laemmli SDS-PAGE of RBC ghost proteins from +/+ (lane 4) and mutant (lanes 1-3) mice. Genotype of mice is indicated above each lane. Size markers are indicated on the left; relative positions of α-spectrin (α) ankyrin (ANK), β-spectrin (β), and band 3 (b3) are indicated on the right. Ratios of α, ANK, and β to b3 are provided in Table 1. (C) Immunoblot of RBC ghost proteins from +/+ (lane 4) and mutant (lanes 1-3) mice. Genotype of mice is indicated above each lane. Primary antibody (1:250 dilution) detects α and β spectrin equally. Size markers are indicated on the left; relative positions of α and β spectrin are indicated on the right.